Accession Number : ADA195076

Title :   Further Studies on Toxin Detection Based on the CO2 Production by Yeast.

Descriptive Note : Final rept. Feb 86-Aug 87,

Corporate Author : NAVAL RESEARCH LAB WASHINGTON DC

Personal Author(s) : Hannan, Patrick J ; Smith, Sekethia L

PDF Url : ADA195076

Report Date : 13 May 1988

Pagination or Media Count : 25

Abstract : Previous studies had shown that certain trichothecene toxins could be detected, in microgram amounts, with baker's yeast as a test organism in a procedure which provided measurements of the CO2 production rates of the test cultures. The present report concerns refinements of the method and the use of a different yeast, Kluyveromyces fragilis, to provide a more sensitive and more rapid detection method for these toxins. The gas exchange method has been modified to include a much higher CO2-stripping rate which greatly decreases the time required to detect the toxins. With the new method the minimum detectable amounts of T-2, roridin, and verrucarin are 0.2, 0.25, and 0.06 micrograms, respectively. The times required for detecting these minimal amounts are somewhat less than an hour; however, for amounts just slightly higher than these the detection times are only 3-5 minutes. Because of the all-or-nothing effects of trichothecenes on the CO2 production rates of yeast, the method is not suitable for making a dose response curve. Its positive aspect is the speed with which it shows the presence of a toxin. Keywords: Yellow rain; Trichothecene toxins; Roridin; Detection methods; T-2 toxins; Verrucarin; Yeast respiration; Mycotoxin; Phytotoxin.

Descriptors :   *PHYTOTOXINS, *TOXICOLOGY, DETECTION, DOSAGE, GAS EXCHANGE(BIOLOGY), GRAPHS, METHODOLOGY, QUICK REACTION, RESPIRATION, RESPONSE(BIOLOGY), YEASTS, YELLOW(COLOR), CARBON DIOXIDE

Subject Categories : Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE