Accession Number : ADA195686

Title :   Use of Tyrosine or Foods to Amplify Catecholamine Release.

Descriptive Note : Final rept. 1 Oct 86-30 Sep 87,

Corporate Author : MASSACHUSETTS INST OF TECH CAMBRIDGE

Personal Author(s) : Wurtman, Richard

PDF Url : ADA195686

Report Date : 05 Feb 1988

Pagination or Media Count : 3

Abstract : Grant funds were used to purchase a multi-detector liquid chromatography system consisting of: BioRad AS-48 refrigerated automatic injector; Hewlett-Packard HP1090 with workstation, software, and fast UV-Vis spectral detector, Berthold LB 506-C radiosotope detector, and an ESA 5100A Coulochem detector. The major equipment has been used to compare several methods of amino acid analysis. At present, we are using pre-column derivitization with OPT with full-spectrum detection. The initial studies have focussed on resolving threonine, glycine, and histidine peaks for a study of CSF samples. Work is beginning on our project on amino acid analysis of retinal superfusates (and, possibly, tissues). (AW)

Descriptors :   *CATECHOLAMINES, *SECRETION, AMINO ACIDS, COMPUTER PROGRAMS, FOOD, GLYCINE, HISTIDINE, RELEASE, TYROSINE, RETINA

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE