Accession Number : ADA195713

Title :   Cloning and Production of Human Acetylcholinesterase.

Descriptive Note : Annual summary rept. May 83-Apr 84,

Corporate Author : ARIZONA UNIV TUCSON DEPT OF MOLECULAR AND CELLULAR BIOLOGY

Personal Author(s) : Aposhian, H V

PDF Url : ADA195713

Report Date : Dec 1984

Pagination or Media Count : 12

Abstract : The goal of this work was to clone by DNA recombinant technology the gene for acetylcholine esterase from a human source. In order to do this we prepared and purified monoclonal antibody from large amounts of medium in which HG-72 hybridoma (ATCC) has been grown. The monoclonal antibody was found to inhibit human acetylcholine esterase but not eel acetylcholine esterase. Attempts to determine and appropriate oligonucleotide sequence probe are also discussed. Keywords: Acetylcholinesterase. (AW)

Descriptors :   ACETYLCHOLINE, ACETYLCHOLINESTERASE, ANTIBODIES, CLONES, ESTERASES, GENETIC ENGINEERING, HUMANS, PRODUCTION, PURIFICATION, SOURCES

Subject Categories : Genetic Engineering and Molecular Biology
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE