Accession Number : ADA195786

Title :   A New Scientific Instrument for Biogenetic Toxicity Testing (UNCL): The Fluorescent Cell Viability Counter (FCVC).

Descriptive Note : Final rept. 15 Sep 87-14 Mar 88,


Personal Author(s) : Benjaminson, M A ; Lehrer, S ; Raie, R R

PDF Url : ADA195786

Report Date : 12 May 1988

Pagination or Media Count : 23

Abstract : On the basis of analyses of commercial technical information and searches of engineering and scientific data bases concomitant with detailed planning sessions and discussions it has been concluded: (1) a SITS-based system will provide the desired capability of discriminating between living and dead cells enabling the measurement of cytotoxicity, (2) the concept of an automated FCVC is feasible (3) commercial cell sorters cannot perform the functional options ascribed to the FCVC, for the most part, and where this capability is claimed, they cannot perform them as cost effectively and efficiently, (4) the relative simplicity of the proposed FCVC environments and reduce the level of training required for operating personnel. The original design concepts have been modified to include replacement of the original double pass sighting system with a more efficient single pass one, an electrostatically controlled flow chamber, an option for segregation and selection of resistant cells for future study an one for one the possible detection and enumeration of specific virus particles. In Phase II, it is planned to build a breadboard/demonstration unit of the prototype FCVC. This unit will be used to study the FCVC's principles of operation, to test and choose components and to determine the operating parameters of the instrument. This will include the development of operating standards and quality control protocols. Keywords: Toxicity, Cells(Biology), Laboratory equipment, Biological stains, Counting methods. (kt)


Subject Categories : Microbiology
      Medicine and Medical Research
      Test Facilities, Equipment and Methods

Distribution Statement : APPROVED FOR PUBLIC RELEASE