Accession Number : ADA195812

Title :   Role of Protein Phosphorylation in the Regulation of Neuronal Sensitivity.

Descriptive Note : Final rept. 1 Sep 84-29 Feb 88,

Corporate Author : VERMONT UNIV BURLINGTON DEPT OF PSYCHIATRY

Personal Author(s) : Ehrlich, Yigal H

PDF Url : ADA195812

Report Date : 28 Apr 1988

Pagination or Media Count : 7

Abstract : Four main directions of investigation have been pursued. Progress has been made in each. First, the endogenous protein phosphorylation systems in various subcellular fractions of neural cells differentiated in-culture have been identified and characterized. Second, we have obtained direct evidence for ecto-protein kinase activity at the surface of neural cells and identified its specific protein substrates. Third, a method for preparing and screening monoclonal antibodies against specific neuronal phosphoproteins has been developed and implemented in our laboratory. Finally, studies of the above systems in primary cultures of brain neurons have been initiated. Several manuscripts describing these novel findings are currently prepared in addition to those already published. Studies on the functional role of protein phosphorylation systems in processes underlying neuronal responsiveness have been initiated during year 02 of this project, using both neural cell lines and primary CNS neurons. In year 03 of the project our research focused on the extracellular protein phosphorylation systems of neostriatral cells differentiated in culture. Keywords: Nerve transmission; Neuronal phosphoproteins; Receptor sensitivity; Ecto-protein kinase. (KT)

Descriptors :   *BRAIN, *NERVE CELLS, *PHOSPHORYLATION, *PROTEINS, ANTIBODIES, CELLS, CENTRAL NERVOUS SYSTEM, CLONES, DOCUMENTS, NERVE TRANSMISSION, NERVOUS SYSTEM, ORIENTATION(DIRECTION), PHOSPHOPROTEINS, REGULATIONS, RESPONSE, SENSE ORGANS, SENSITIVITY, SUBSTRATES

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE