Accession Number : ADA195939

Title :   Interactions of Glial Proteins from the Regenerating Goldfish (C. auratus) Optic Nerve with Retinotectal Axons,

Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s) : Deaton, Michael A ; Bock, Susan E ; Freeman, John A

PDF Url : ADA195939

Report Date : Jun 1988

Pagination or Media Count : 15

Abstract : Three proteins, with apparent molecular weights of 16, 30, and 42 kilodaltons, made by glial cells of the goldfish optic nerve undergo enhanced synthesis during regeneration of the retinotectal axons. If, as has been postulated, these enhanced glial proteins (or EGPs) assist in axonal regeneration, they may interact with the retinotectal neurons. Two possible modes of interaction, 1) endocytosis/retrograde transport and 2) binding to optic nerve membranes, were examined using gel electrophoresis of radiolabelled glial proteins. The results suggest 1) that two glial proteins, which may be EGP-16 and EGP-42, are retrogradely transported to the retina during axonal regeneration and 2) that a third glial protein, perhaps EGP-30, binds in a specific and saturable manner to the membranous fraction of the optic nerve. Keywords: Glial cells; Nerve regeneration; Retrograde transport; Protein synthesis; Protein binding; neurogenesis. (kt)

Descriptors :   BIOSYNTHESIS, ELECTROPHORESIS, GELS, INTERACTIONS, MEMBRANES, MOLECULAR WEIGHT, NERVE CELLS, NERVE FIBERS, NERVES, OPTIC NERVE, PROTEINS, REGENERATION(ENGINEERING), REGENERATION(PHYSIOLOGY), RETINA, SYNTHESIS

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE