Accession Number : ADA195949

Title :   Gene Regulation of Methanogenesis from Acetate in the Acetotrophic Methane Producing Archaebacteria.

Descriptive Note : Final rept. Mar 86-Jun 88,

Corporate Author : CALIFORNIA UNIV LOS ANGELES DEPT OF MICROBIOLOGY

Personal Author(s) : Gunsalus, Robert P

PDF Url : ADA195949

Report Date : 27 Jun 1988

Pagination or Media Count : 10

Abstract : The research objectives of the project were to develop genetic and molecular techniques that will permit the detection, isolation, and cloning of genes that are regulated during acetate catabolism in the Methanosarcina spp. These studies should provide a basis for examining the regulation of acetate utilization by the methanogenic Methanosarcina. We have concentrated on three areas in the two year period of the contract. They are: 1) refining plating techniques and selecting for auxotrophic mutants; 2) characterizing plasmid(s) from Methanosarcina spp.; 3) creating a gene library from Methanosarcina spp.; 4) creating oligonucleotide and antibody probes and probing the chromosomal library for crossover dehydrogenase genes. (AW)

Descriptors :   *ACETATES, *CATABOLISM, *GENES, *MOLECULAR BIOLOGY, ANTIBODIES, CHROMOSOMES, CLONES, DETECTION, GENETIC ENGINEERING, GENETICS, ISOLATION, METHANE, PLATING, PROBES, NUCLEOTIDES, CHROMOSOMES, DEHYDROGENASES

Subject Categories : Genetic Engineering and Molecular Biology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE