Accession Number : ADA195969

Title :   Lipopolysaccharide Antigens of Pseudomonas aeruginosa and Design of Novel Vaccines.

Descriptive Note : Final rept. (Annual) 1 Sep 84-30 Apr 86,

Corporate Author : OHIO STATE UNIV RESEARCH FOUNDATION COLUMBUS

Personal Author(s) : Horton, Derek

PDF Url : ADA195969

Report Date : Sep 1987

Pagination or Media Count : 28

Abstract : The antigenic lipopolysaccharides (LPS) of the cell coats of five of the seven Fisher immunotype strains of the Gram-negative pathogen Pseudomonas aeruginosa have been characterized structurally by chemical methods involving degradation techniques, and by physical methods, notably high-field NMR spectroscopy. Of the seven Fisher types, the O-Chain structures of five of the types have been characterized in our laboratories. Partial structures for the remaining two types have been elucidated. The Gram-negative bacterium Pseudomonas aeruginosa is ubiquitous in its occurrence and is characterized by a very wide variety of strains as determined by conventional serotyping methods. Although healthy individuals normally have a high innate resistance to infection by Pseudmonas aeruginosa as a result of effective combat by matured antibodies, the organism is an opportunistic pathogen; virulent strains lead to dangerous, often fatal, infections in human subjects whose natural resistance has been lowered. Conventional treatment with antibiotics effective against Gram-positive organisms is of little value, as such therapeutic agents are unable to penetrate the lipopolysaccharide layer that forms a thick, hydrophobic outer coating of the cells in Gram-negative organisms. In view of the limited effectiveness of conventional antibiotic agents for combat of Pseudomonas infections, immunotherapeutic methods have been explored as an alternative method. Keywords: Burns; cystic fibrosis. (kt)

Descriptors :   ANTIBIOTICS, ANTIBODIES, ANTIGENS, BACTERIA, CHEMICALS, CHEMOTHERAPEUTIC AGENTS, COATINGS, CYSTS, DEGRADATION, DISEASES, EXTERNAL, FIBROSIS, GRAM NEGATIVE BACTERIA, HYDROPHOBIC PROPERTIES, INFECTIOUS DISEASES, LAYERS, LIPOPOLYSACCHARIDES, PHYSICAL PROPERTIES, PSEUDOMONAS, PSEUDOMONAS AERUGINOSA, RESISTANCE, SPECTROSCOPY, STRAINS(BIOLOGY), STRUCTURES, THICKNESS, VACCINES, VIRULENCE, WARFARE

Subject Categories : Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE