Accession Number : ADA196772

Title :   Total Triiodothyronine by Fluorescence Polarization Immunoassay (FPIA),

Corporate Author : SCHOOL OF AEROSPACE MEDICINE BROOKS AFB TX

Personal Author(s) : Armbruster, Dave ; Harris, Roderick ; Scarbrough, Rhonda ; Tamez, Carlos

Report Date : 1988

Pagination or Media Count : 4

Abstract : The determination of total triiodothyronine (T3) is a useful adjunct to the more common thyroid function battery consisting of total thyroxine (T4), T3 uptake (T3U), and thyroid stimulating hormone, (TSH). Elevated total T3 values are indicative of hyperthyroid conditions such as T3 toxicosis or Graves' disease. Traditionally, radioimmunoassays (RIA) have been employed for the determination of total T3. Enzyme immunoassays (EIA) and fluorescence immunoassays (FIA) have been developed for many of the analytes that formerly were measured using RIA. One variation of this new generation of immunoassays is fluorescence polarization. A fluorescence polarization immunoassay (FPIA) method for total T3 has been automated by adaptation to the TDx (Abbott, Chicago, IL) clinical analyzer. The TDx total T3 assay has been evaluated as a replacement for an RIA total T3 procedure. Performance characteristics of the FPIA test are reported here along with comparisons and contrasts with the more commonly used RIA test. Reprints. (MJM)

Descriptors :   *FLUORESCENCE, *IMMUNOASSAY, *POLARIZATION, *THYROXINE, ADAPTATION, ANALYZERS, AUTOMATION, CLINICAL MEDICINE, ENZYMES, RADIOIMMUNOASSAY, REPRINTS, VARIATIONS.

Subject Categories : Biochemistry
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE