Accession Number : ADA200556

Title :   A Core Facility for the Study of Neurotoxins of Biological Origin.

Descriptive Note : Annual rept. 15 May 87-14 May 88,

Corporate Author : JEFFERSON MEDICAL COLL PHILADELPHIA PA DEPT OF MEDICINE

Personal Author(s) : Simpson, Lance L.

Report Date : 15 JUN 1988

Pagination or Media Count : 156

Abstract : The toxin of major interest have been gyroxin, tetanus toxin, botulinum neurontoxin, the botulinum binary toxin, and ryanodine. Experiments have been conducted mainly on excised tissues, such as the phrenic nerve-hemidiaphragm preparation, and on cells in tissue culture, including neuroblastoma cells and adrenal medullary tumor cells. The major accomplishments have been: i.) the development of a rapid method for purifying gyroxin, ii.) characterization of the effects of gyroxin on central nervous system metabolism, iii.) a further description of the effects of ryanodine on divalent cation movement and oxygen utilization in muscle cells, iv.) an exhaustive evaluation of the effects of tetanus toxin on intracellular messenger systems, v.) a description of the pharmacological interaction between various drugs and botulinum neurotoxin, vi.) isolation and characterization of a human monoclonal antibody against tetanus toxin. Keywords: Neurophysiology, Central Nervous System. (KT/AW)

Descriptors :   *BACTERIAL TOXINS, *CENTRAL NERVOUS SYSTEM, *PHARMACOLOGY, *TOXINS AND ANTITOXINS, ALKALOIDS, ANTIBODIES, CATIONS, CELLS, CLOSTRIDIUM BOTULINUM, CORES, DRUGS, FACILITIES, HUMANS, INTERACTIONS, ISOLATION, METABOLISM, MUSCLES, NERVOUS SYSTEM, NEUROBLASTOMA, NEUROPHYSIOLOGY, OXYGEN, REPRODUCTION(PHYSIOLOGY), TETANUS, TISSUE CULTURE, UTILIZATION, VALENCE.

Subject Categories : Biochemistry
      Toxicology
      Pharmacology

Distribution Statement : APPROVED FOR PUBLIC RELEASE