Accession Number : ADA201847

Title :   Production and Characterization of Monoclonal Antibodies to the Protective Antigen Component of Bacillus anthracis Toxin,

Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s) : Little, Stephen F. ; Leppla, Stephen H. ; Cora, Elsa

Report Date : JUL 1988

Pagination or Media Count : 7

Abstract : Thirty-six monoclonal antibodies to the protective antigen of Bacillus anthracis exotoxin have been characterized for affinity, antibody subtype, competitive binding to antigenic regions, and ability to neutralize lethal and edema toxin activities. At least 23 antigenic regions were detected on protective antigen by a blocking, enzyme-linked immunosorbent assay. Two clones, 3B6 and 14B7, competed for a single antigenic region and neutralized the activity of both the lethal toxin in vivo (Fisher 344 rat) and the edema toxin in vitro (CHO cells). These two antibodies blocked the binding of (125) I-labeled protective antigen to FRL-103 cells. Our results support the proposal that binding of protective antigen to cell receptors is required for expression of toxicity. Keywords: Toxins and antitoxins; Binding sites; Reprints. (kt)

Descriptors :   *ANTIBODIES, *ANTIGENS, *BACILLUS ANTHRACIS, *TOXINS AND ANTITOXINS, CLONES, EDEMA, ENZYMES, EXOTOXINS, IMMUNOASSAY, IN VITRO ANALYSIS, IN VIVO ANALYSIS, LETHALITY, PRODUCTION, REGIONS, REPRINTS, TOXICITY.

Subject Categories : Pharmacology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE