Accession Number : ADA204591

Title :   Sister Chromatid Exchange Assay of Nitrosoguanidine in Chinese Hamster Ovary Cells.

Descriptive Note : Rept. for 9 Sep 85-12 Nov 86,

Corporate Author : LETTERMAN ARMY INST OF RESEARCH PRESIDIO OF SAN FRANCISCO CA

Personal Author(s) : Harbell, John W. ; Witcher, Lillie D. ; Korte, Don W., Jr ; Wheeler, Conrad R.

Report Date : DEC 1988

Pagination or Media Count : 17

Abstract : Nitrosoguanidine is a potential anaerobic degradation product of nitroguanidine, a primary component of US Army triple-base propellants. The potential of nitrosoguanidine to induce Sister Chromatid Exchanges (SCEs) was assessed using Chinese Hamster Ovary (CHO) cells both with and without exogenous metabolic activation provided by rat liver S-9. Two assays were performed. In the first assay, cells were exposed to test compound concentrations ranging from 1 mg/ml to 0.01 mg/ml in cultures with and without exogenous metabolic activation. In the second assay, cells were exposed to doses of 1.5 to 1.1 mg/ml without activation and 1.35 to 1.0 mg/ml with activation. Nitrosoguanidine induced a statistically significant increase in SCEs both with and without exogenous metabolic activation. The positive response occurred at the upper limits of toxicity in cultures without metabolic activation. In cultures with metabolic activation, the top doses produced a positive response but no correlated dose-dependent increase in SCEs. These results indicate that nitrosoguanidine induced a significant increase in the number of SCEs under the conditions of this study. Keywords: DNA damage, Genetic toxicology. (aw)

Descriptors :   *NITROGUANIDINE, *OVARIES, *TOXICITY, *CHROMOSOMES, ACTIVATION, ANAEROBIC PROCESSES, ARMY EQUIPMENT, BIOASSAY, CELLS(BIOLOGY), CHROMATIN, DAMAGE, DEGRADATION, DEOXYRIBONUCLEIC ACIDS, DOSAGE, EXCHANGE, GENETICS, HAMSTERS, METABOLISM, MULTIBASE PROPELLANTS, PROPELLANTS, RESPONSE(BIOLOGY), TOXICOLOGY.

Subject Categories : Toxicology
      Ammunition and Explosives

Distribution Statement : APPROVED FOR PUBLIC RELEASE