Accession Number : ADA226504
Title : Autocrine Stimulation of Interleukin-1(alpha) and Transforming Growth Factor(alpha) Production in Human Keratinocytes and Its Antagonism by Glucocorticoids,
Corporate Author : STANFORD UNIV CA
Personal Author(s) : Lee, Simon W. ; Morhenn, Vera B. ; Ilnicka, Mariola ; Eugui, Elsie M. ; Allison, Antnony C.
Report Date : 1989
Pagination or Media Count : 22
Abstract : Interleukin-1 (IL-1) and transforming growth factor alpha (TGF alpha) mRNA expression was analysed in cultured normal human keratinocytes. Keratinocytes constitively express IL-1 mRNA when cultured in keratinocyte growth medium but not in Dulbecco's minimal essential medium containing fetal bovine serum, in which the cells differentiate. The predominant form of IL-1 expressed by keratinocytes is IL-1 alpha. Addition of IL-1 alpha to keratinocytes increased IL-1 alpha and TGF alpha mRNA expression in a dose-dependent manner. TGF alpha induced a similar increase in IL-1 alpha and TGF alpha mRNAs in keratinocytes. Hydrocortisone decreased the expression of both IL-1 alpha and TGF alpha mRNAs in keratinocytes. These findings document an autocrine mechanism by which IL-1 alpha and TGF alpha can stimulate the proliferation of keratinocytes in the skin. Acquired immune deficiency syndrome. (js)
Descriptors : *MEDICINE, *ACQUIRED IMMUNE DEFICIENCY SYNDROME, GROWTH SUBSTANCES, BIOCHEMISTRY.
Subject Categories : Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE