Accession Number : ADA226504

Title :   Autocrine Stimulation of Interleukin-1(alpha) and Transforming Growth Factor(alpha) Production in Human Keratinocytes and Its Antagonism by Glucocorticoids,

Corporate Author : STANFORD UNIV CA

Personal Author(s) : Lee, Simon W. ; Morhenn, Vera B. ; Ilnicka, Mariola ; Eugui, Elsie M. ; Allison, Antnony C.

Report Date : 1989

Pagination or Media Count : 22

Abstract : Interleukin-1 (IL-1) and transforming growth factor alpha (TGF alpha) mRNA expression was analysed in cultured normal human keratinocytes. Keratinocytes constitively express IL-1 mRNA when cultured in keratinocyte growth medium but not in Dulbecco's minimal essential medium containing fetal bovine serum, in which the cells differentiate. The predominant form of IL-1 expressed by keratinocytes is IL-1 alpha. Addition of IL-1 alpha to keratinocytes increased IL-1 alpha and TGF alpha mRNA expression in a dose-dependent manner. TGF alpha induced a similar increase in IL-1 alpha and TGF alpha mRNAs in keratinocytes. Hydrocortisone decreased the expression of both IL-1 alpha and TGF alpha mRNAs in keratinocytes. These findings document an autocrine mechanism by which IL-1 alpha and TGF alpha can stimulate the proliferation of keratinocytes in the skin. Acquired immune deficiency syndrome. (js)


Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE