Accession Number : ADA245397

Title :   Role of Protein Methylation in Halobacterium halobium Phototaxis.

Descriptive Note : Final rept. 1 Feb 89-1 Jul 91,

Corporate Author : ALBERT EINSTEIN COLL OF MEDICINE BRONX NY

Personal Author(s) : Spudich, John L.

Report Date : 21 JAN 1992

Pagination or Media Count : 4

Abstract : The objective of this grant was to investigate the role of methyl-accepting proteins in the phototaxis signaling system of H. halobium membranes. A carboxylmethylated protein in the membrane, MPP-I (Methyl-accepting phototaxis protein I) appears to relay the signal from photoactivated sensory rhodopsin I (SR-I, a visual pigment-like photosensor). Our primary objective was to elucidate the relationship between SR-I and MPP-I. Accomplishments in this period have been in 3 areas: (1) Signaling conformations of SR-I have been studied and processes important to receptor activation identified; (2) Tryptic peptide sequence has been obtained from MPP-I and the gene encoding MPP-I cloned; and (3) in collaboration with the laboratory of H. G. Khorana, we have designed, synthesized and expressed an SR-I gene optimized for site-directed mutagenesis.

Descriptors :   ACTIVATION, EYE PIGMENTS, METHYLATION, PHOTOACTIVATION ANALYSIS, PROTEINS, SENSE ORGANS, SENSES(PHYSIOLOGY), SIGNALS.

Subject Categories : Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE