Accession Number : ADA245397
Title : Role of Protein Methylation in Halobacterium halobium Phototaxis.
Descriptive Note : Final rept. 1 Feb 89-1 Jul 91,
Corporate Author : ALBERT EINSTEIN COLL OF MEDICINE BRONX NY
Personal Author(s) : Spudich, John L.
Report Date : 21 JAN 1992
Pagination or Media Count : 4
Abstract : The objective of this grant was to investigate the role of methyl-accepting proteins in the phototaxis signaling system of H. halobium membranes. A carboxylmethylated protein in the membrane, MPP-I (Methyl-accepting phototaxis protein I) appears to relay the signal from photoactivated sensory rhodopsin I (SR-I, a visual pigment-like photosensor). Our primary objective was to elucidate the relationship between SR-I and MPP-I. Accomplishments in this period have been in 3 areas: (1) Signaling conformations of SR-I have been studied and processes important to receptor activation identified; (2) Tryptic peptide sequence has been obtained from MPP-I and the gene encoding MPP-I cloned; and (3) in collaboration with the laboratory of H. G. Khorana, we have designed, synthesized and expressed an SR-I gene optimized for site-directed mutagenesis.
Descriptors : ACTIVATION, EYE PIGMENTS, METHYLATION, PHOTOACTIVATION ANALYSIS, PROTEINS, SENSE ORGANS, SENSES(PHYSIOLOGY), SIGNALS.
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE