Accession Number : ADA255156

Title :   Assessment of DNA Modifications in Medaka (0. Latipes) Exposed to Chemicals in Contaminated Ground Water.

Descriptive Note : Final rept. 30 Sep 88-29 Oct 91,

Corporate Author : PACIFIC NORTHWEST RESEARCH FOUNDATION SEATTLE WA

Personal Author(s) : Malins, Donald C.

Report Date : 31 OCT 1991

Pagination or Media Count : 51

Abstract : A method has been developed for the analysis of oxidative modifications in the DNA of Medaka based on gas chromatography-mass spectrometry, coupled with selected ion monitoring. This sensitive method allows for the structural and quantitative determination of oxidative base changes in fish exposed to low concentrations of groundwater contaminants and other chemicals. Overall it is potentially useful for elucidating genotoxic injury in animals exposed to environmental chemicals at hazardous waste sites.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, ANIMALS, CHEMICALS, CHROMATOGRAPHY, CONTAMINANTS, DETERMINATION, FISHES, GAS CHROMATOGRAPHY, GROUND WATER, IONS, LESIONS, MASS, MASS SPECTROMETRY, MODIFICATION, MONITORING, SITES, SPECTROMETRY, WASTES, WATER, WOUNDS AND INJURIES, OXIDES, ENVIRONMENTS, CHEMICALS, HAZARDOUS MATERIALS, TOXIC HAZARDS, CARCINOGENS.

Subject Categories : Medicine and Medical Research
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE