Accession Number : ADA258586

Title :   Pathobiology of HIV in the Human Monocyte-Macrophage.

Descriptive Note : Annual rept. 28 Sep 91-27 Sep 92,

Corporate Author : NEW ENGLAND DEACONESS HOSPITAL BOSTON MA

Personal Author(s) : Groopman, Jerome E.

Report Date : 13 OCT 1992

Pagination or Media Count : 47

Abstract : In the past year, we have made considerable progress in addressing specific aims of our proposal. We have studied the interaction of HIV with monocyte-macrophages and compared these interactions with those that occur in human T lymphocytes. We have determined that human kit ligand/stem cell factor, a newly identified cytokine elaborated by mesenchymal cells, does not up-regulate HIV infection in monocyte-macrophages and is capable of protecting hematopoietic progenitors from a number of suppressive cytokines and drugs. Human interleukin-3 was found to be less active in protecting similar populations of cells and is known to up-regulate HIV, making the kit ligand/stem cell factor a cytokine whose therapeutic potential should be further explored in the context of AIDS. We have also pursued the regulation of HIV expression by steroid hormone receptors. It appears that there is a responsive element in the negative regulatory element (NRE) of many, but not all, HIV isolates which bind homodimers and heterodimers of different steroid hormone receptor proteins. The response of monocytes to physiological ligands such as retinoids, with respect to modulation of HIV infection, is under investigation.

Descriptors :   *HUMAN IMMUNODEFICIENCY VIRUSES, *HUMANS, *MACROPHAGES, *MONOCYTES, CELLS, DRUGS, HORMONES, INTERACTIONS, KITS, LIGANDS, LYMPHOCYTES, POPULATION, PROTEINS, RESPONSE, STEROIDS, T LYMPHOCYTES, PATHOLOGY, ACQUIRED IMMUNE DEFICIENCY SYNDROME, IN VITRO ANALYSIS, IN VIVO ANALYSIS, STRAINS(BIOLOGY), CLONES, FIBROBLASTS, PATIENTS.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE