Accession Number : ADA259938

Title :   Electron Conducting Adducts of Water-Soluble Redox Polyelectrolytes and Enzymes.

Descriptive Note : Technical rept. no. 1, Oct 92-Jan 93,

Corporate Author : TEXAS UNIV AT AUSTIN DEPT OF CHEMICAL ENGINEERING

Personal Author(s) : Katakis, Ioanis ; Vreeke, Mark ; Ye, Ling ; Aoki, Atsushi ; Heller, Adam

Report Date : 26 JAN 1993

Pagination or Media Count : 39

Abstract : Biochemical fluxes were transduced to electrical currents through catalytic centers of oxidoreductases, that were electrically connected to electrodes with crosslinked hydrogel forming redox polymers. The enzymes were covalently bound to the hydrogels through which the electrons diffuse. The electron diffusion coefficients, reaching 10-8cm2sec-1 in the redox polymer gels, were high enough to allow efficient collection of the electrons transferred during the electrooxidation or electroreduction of substrates. ne adequate electron diffusion through the polymer skeleton of the hydrogel and the rapid diffusion of water-soluble substrates and products in the gels led to high current density enzyme electrodes. These included electrodes with electrically connected flavoenzymes (e.g. sarcosine, glutamate, lactate and glucose oxidases), quinoprotein enzymes (e.g. PQQ glucose dehydrogenase) and heme enzymes (e.g. peroxidases). The peroxidase electrodes are useful in the sensing of NAD(P)H, that catalytically reacts with oxygen in the presence of N-methyl phenazonium salts to form NAD(P)+ and H202.... Redox, Polyelectrolytes, Enzymes, Flavoenzymes, Quinoprotein enzymes, Heme enzymes, NAD(P)H, NAD(P)+ and H2O2.

Descriptors :   *ELECTRONS, *ENZYMES, *POLYELECTROLYTES, *CONDUCTIVITY, COEFFICIENTS, COLLECTION, CURRENT DENSITY, DEHYDROGENASES, DENSITY, DIFFUSION, ELECTRODES, GELS, GLUCOSE, HEMOGLOBIN, LACTATES, OXIDOREDUCTASES, OXYGEN, PEROXIDASES, POLYMERS, SALTS, SKELETON, SUBSTRATES, WATER, SOLUBILITY, OXIDATION REDUCTION REACTIONS, FLUX(RATE), BIOCHEMISTRY, TRANSDUCERS, CATALYSIS, CROSSLINKING(CHEMISTRY), COVALENT BONDS, PROTEINS.

Subject Categories : Biochemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE