Accession Number : ADA278763

Title :   Synthesis of Novel Composite Platinum-DNA Films via Wet-Spinning.

Descriptive Note : Final rept. 1 Mar 91-28 Feb 94,

Corporate Author : TOLEDO UNIV OH DEPT OF PHYSICS AND ASTRONOMY

Personal Author(s) : Lee, Scott A.

Report Date : 18 APR 1994

Pagination or Media Count : 127

Abstract : In this work a variety of novel films were produced. The films consisted of DNA complexed with the platinum compounds ((bipy)Pt(en)) and cis-diamminedichloroplatinum(II) as well as netropsin and daunomycin. Other films were made of either DNA or hyaluronic acid (HA) with different metallic counterions. These films were studied in order to determine if the films had enhanced optical or acoustical properties. Optical, third harmonic and second harmonic generation were observed from DNA, DNA/bipy, and DNA/netropsin films. An optical anomaly was also observed at the A-to-B transition in CsDNA. Enhanced acoustic properties were found in Li- and NaHA: both samples have a speed of sound of about 5 km/s at low relative humidity (RH). This stiffness appears to be related to the strong Coulombic bonding involving the metallic counterions. The HA films also display a phase transition in the vicinity of 88% RH, as revealed by Brillouin and Raman spectroscopy, infrared absorption, ultraviolet absorption and optical polarizabilities. Films of DNA complexes also show enhanced acoustical properties. In particular, DNA/netropsin films show a dramatic stiffening of the bonding holding the two helices together. Enhanced optical and acoustical properties, DNA, DNA/ligand, Hyaluronic films

Descriptors :   *FILMS, *POLYMERS, *NONLINEAR OPTICS, *DEOXYRIBONUCLEIC ACIDS, ABSORPTION, ACIDS, ACOUSTIC PROPERTIES, ACOUSTICS, ANOMALIES, BONDING, HARMONICS, HUMIDITY, LIGANDS, PHASE, PHASE TRANSFORMATIONS, PLATINUM, PLATINUM COMPOUNDS, RAMAN SPECTROSCOPY, SECOND HARMONIC GENERATION, SOUND, SPECTROSCOPY, STIFFENING, STIFFNESS, TRANSITIONS, VELOCITY, WORK, SYNTHESIS(CHEMISTRY), HYALURONATE LYASE, MODELS, POLYMERIC FILMS.

Subject Categories : Genetic Engineering and Molecular Biology
      Organic Chemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE