Accession Number : ADA279774

Title :   In Vivo Distribution of Liposome Encapsulated Hemoglobin Studied With Imaging Radiotracers.

Descriptive Note : Final technical rept. 1 Dec 90-31 Mar 94,

Corporate Author : TEXAS UNIV HEALTH SCIENCE CENTER AT SAN ANTONIO DEPT OF RADIOLOGY

Personal Author(s) : Phillips, William T.

Report Date : 31 MAR 1994

Pagination or Media Count : 117

Abstract : The objective of this project was to develop radiotracer imaging technology to follow the in vivo circulation and organ deposition of liposome encapsulated hemoglobin (LEH). A liposome labeling technique based on the most common radioisotope used in nuclear medicine, technetium-99m (99m Tc), was developed and patented. A comparison of this 99mTc-liposome labeling method with previous methods using indium-111 (111 In) was performed. The label LEH was infused into small animals to monitor any in vivo differences between different LEH formulations (bovine hemoglobin, recombinant human hemoglobin, human hemolysate, lyophilized) using gamma scintigraphy and tissue biodistribution determination. These studies were correlated with any hematological and pathological changes associated with LEH treatment such as the interaction of LEH with circulating platelets using indium-111-label platelets

Descriptors :   *LIPOSOMES, *IN VIVO ANALYSIS, *ENCAPSULATION, *HEMOGLOBIN, NUCLEAR MEDICINE, ANIMALS, BOVINES, OXYGEN, HUMANS, CELLS, BRAIN, TEMPERATURE, REPORTS.

Subject Categories : Anatomy and Physiology
      Mfg & Industrial Eng & Control of Product Sys
      Biochemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE