Accession Number : ADA282032

Title :   Cytogenetic Studies of testicular Germ Cell Tumors with Chromosome 12 Specific DNA Probes.

Descriptive Note : Doctoral thesis,

Corporate Author : AIR FORCE INST OF TECH WRIGHT-PATTERSON AFB OH

Personal Author(s) : Reilly, Patricia A.

Report Date : DEC 1993

Pagination or Media Count : 185

Abstract : The usefulness of interphase cytogenetics in the study of testicular germ cell tumors (TGCT) was assessed using fluorescence in situ hybridization (FISH) and a chromosome 12 specific alpha-satellite probe. Nuclei isolated from paraffin-embedded blocks from 79 patients with stage III TGCT were studied after FISH. The overall number, number of large (chromosome 12) and small I(12P) signals, and total signals were measured in hyperdiploid tumor nuclei. These was no evidence of difference in overall signal number or size between seminomas and nonseminomas or among tumor histologies, nor was there evidence of an association between any of the above signal parameters and patient outcome. A ratio of the number of copies of 12p (small signals) to the number of copies of chromosome 12 (large signals) appeared to be predictive for patient outcome. As this ratio increased, survival time decreased.

Descriptors :   *GERM CELLS, *CANCER, *DEOXYRIBONUCLEIC ACIDS, CHROMOSOMES, NUCLEI, EMBEDDING, PATIENTS, HISTOLOGY.

Subject Categories : Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE