Accession Number : ADA285641

Title :   Monoclonal Antibody Detection of Chlorinated Benzenes on Contaminated Sediments.

Descriptive Note : Final technical rept. 1 May 91-31 Jul 94,

Corporate Author : MISSOURI UNIV-COLUMBIA DEPT OF CIVIL ENGINEERING

Personal Author(s) : Mossman, Deborah J. ; Feldbush, Thomas L.

Report Date : 30 SEP 1994

Pagination or Media Count : 39

Abstract : A modification to allow direct testing of soils and sediments has been made to the standard immunoassay procedure. The modified procedure eliminates the need for extraction prior to ELISA testing. The new method has been successfully tested using 2,4-dinitrobenzene sulfonate as the model pollutant and crushed brick and sand as model soil matrices. The modified ELISA is very sensitive and easily distinguishes between contamination levels. Monoclonal antibodies were produced from antigens created from 4-chloroaniline, 2,4-dichloroaniline, and 3,4-dichloroaniline. One cell line of the anti-4-chloroaniline antibodies reacts to 4-chloroaniline, 2,4,5-trichloraniline, and 2,4,5-trichlorophenol. Immunoassay, Sediment testing, ELISA

Descriptors :   *CONTAMINATION, *IMMUNOASSAY, *MONOCLONAL ANTIBODIES, ANTIBODIES, ANTIGENS, BRICK, CELLS, EXTRACTION, MODELS, MODIFICATION, POLLUTANTS, SAND, SEDIMENTS, SOILS, STANDARDS, SULFONATES, CHLORINATED HYDROCARBONS, BENZENE, TEST AND EVALUATION, MATRICES(MATHEMATICS), PROTEINS, CROSSLINKING(CHEMISTRY), CULTURES(BIOLOGY).

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE