Accession Number : ADA288525

Title :   Voltammetric and Amperometric Probes for Single Cell Analysis.

Descriptive Note : Technical rept.,

Corporate Author : PENNSYLVANIA STATE UNIV UNIVERSITY PARK DEPT OF CHEMISTRY

Personal Author(s) : Ewing, Andrew G. ; Chen, Ta K.

PDF Url : ADA288525

Report Date : 26 MAR 1993

Pagination or Media Count : 63

Abstract : The use of voltammetry and amperometry to monitor chemical dynamics at single nerve cells and nerve cell model systems has provided information about intracellular neurotransmitter dynamics and a means to directly monitor exocytosis. It is important, however, to emphasize that these techniques can as yet be used only for easily oxidized neurotransmitters and concentration sensitivity is an issue. It is not yet possible to voltammetrically detect submicromolar levels of catecholamine in the cytoplasm of a cell. Finally, these techniques have as yet only been used on cells in culture and large identified cells in invertebrates. A large step in technology is needed to fabricate smaller electrode sizes and manipulation techniques before voltammetric measurements will be made in individual synapses.

Descriptors :   *VOLTAMMETRY, *IN VIVOANAYLSIS, SIZES(DIMENSIONS), MODELS, DYNAMICS, CELLS, OXIDATION, ELECTRODES, CHEMICAL REACTIONS, NERVE CELLS, CYTOPLASM, CATECHOLAMINES, CELLS(BIOLOGY), INVERTEBRATES, NEUROTRANSMITTERS, SYNAPSE, ELECTRON MICROSOPES, IMMUNOASSAY.

Subject Categories : Anatomy and Physiology
      Electricity and Magnetism

Distribution Statement : APPROVED FOR PUBLIC RELEASE