Accession Number : ADA289837

Title :   Development of an Aquatic Bioassay using the Medaka (Oryzias latipes) to Assess Human Health Risk: Tumor Immunodiagnosis.

Descriptive Note : Final rept. 28 Sep 90-27 Feb 94,

Corporate Author : JOHNS HOPKINS UNIV BALTIMORE MD SCHOOL OF MEDICINE

Personal Author(s) : Bunton, Tracie E.

PDF Url : ADA289837

Report Date : 10 JAN 1995

Pagination or Media Count : 25

Abstract : In a continuation project for the development of aquatic bioassays as alternatives for carcinogenicity and toxicity testing to assess human health risk, immunohistochemical techniques (IHC) for the diagnosis of carcinogen induced neoplasms and other proliferative lesions in fish will be developed. Groups of medaka (Oryzias latipes have been exposed to either N-methyl-N'-nitro-nitrosoguanidine (MNNG) or methylazoxymethanol acetate (MAN-Ac) to induce variable neoplasms and proliferative lesions to which IHC can be applied to identify differentiation antigens. Both peroxidase-antiperoxidase and avidin biotin complex techniques are used to identify intermediate filament proteins keratin, desmin, vimentin, glial fibrillary acidic protein, and neurofilament protein. Other selected antigens may also be tested for based on histology. These findings will be correlated with histology, and ultrastructure. The results of this proposal will identify association between cell types in neoplasms and in proliferative lesions of uncertain origin which cannot yet be types by morphology alone in the fish, and thereby identify the possible progression of these lesions to neoplasms. These data are essential to the development of aquatic bioassays to evaluate human health risk and thus this research will contribute significantly to the development of the model.

Descriptors :   *TOXICITY, *DIAGNOSIS(MEDICINE), *VERTEBRATES, *IMMUNOLOGY, TEST AND EVALUATION, RISK, MODELS, HUMANS, HEALTH, PROTEINS, NEOPLASMS, VARIABLES, CARCINOGENS, MEDICAL SERVICES, AQUATIC ORGANISMS, ANTIGENS, LESIONS, HISTOLOGY, ACIDS, BIOASSAY.

Subject Categories : Medicine and Medical Research
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE