Accession Number : ADA290155

Title :   Services to Develop, Standardize, and Validate Polymerase Chain Reaction (PCR) Protocols for the Detection of Leishmaniasis in Clinical Samples.

Descriptive Note : Final rept. Sep 92-Sep 94,

Corporate Author : SRA TECHNOLOGIES INC FALLS CHURCH VA

Personal Author(s) : White, Frank A., III ; Suryanaarayana, Kalachar

PDF Url : ADA290155

Report Date : 17 OCT 1994

Pagination or Media Count : 127

Abstract : The disease Leishmaniasis, endemic in Africa, South West Asia, and South America, is caused by transmission of a parasite of the Theishmania species via the bite of an infected sandfly. The severity of the disease ranges from cutaneous lesions to a frequently fatal visceralization of the internal organs unless treated at an early stage. Detection of the parasite is difficult because only low numbers of infected cells are found in Peripheral blood. Existing tests for Theishmania parasites are time consuming and have high (50%) false negative rates. We report here the development of a rapid polymerase chain reaction (PCR) based diagnostic capable of detecting 1-10 infected cells in 1.5 x 10 6 PBMCs or 2-5 mis of peripheral blood. The test has an accuracy greater than 92%, and a false negative rate of less than 8%, when validated against known clinical samples.

Descriptors :   *POLYMERIZATION, *CHAIN REACTIONS, *LEISHMANIASIS, QUICK REACTION, DETECTION, RATES, CLINICAL MEDICINE, DISEASES, ACCURACY, DIAGNOSIS(MEDICINE), INTERNAL, MILITARY MEDICINE, SAMPLING, PARASITES, MEDICAL SERVICES, AFRICA, NUMBERS, WEST(DIRECTION), PATHOGENESIS, BLOOD, SOUTH AMERICA, LESIONS, ORGANS(ANATOMY), SKIN DISEASES, SOUTH ASIA, ZOONOTIC DISEASES.

Subject Categories : Polymer Chemistry
      Microbiology
      Nuclear Physics & Elementary Particle Physics

Distribution Statement : APPROVED FOR PUBLIC RELEASE