Accession Number : ADA290439

Title :   Biosensors Based on Cross-Linking of Biotinylated Glucose Oxidase By Avidin.

Descriptive Note : Technical rept. 1 Jan 94-25 Jan 95,

Corporate Author : TEXAS UNIV AT AUSTIN DEPT OF CHEMICAL ENGINEERING

Personal Author(s) : Vreeke, Mark S. ; Rocca, Patrick

PDF Url : ADA290439

Report Date : 19 JAN 1995

Pagination or Media Count : 20

Abstract : Avidin, having four biotin binding units, cross-links and immobilizes glucose oxidase (GOX) labeled with multiple biotins (B-GOX) when solutions of B-GOX and avidin are mixed on an electrode. The H2O2 flux generated in the B-GOX catalyzed oxidation of glucose by dissolved O2 is measured as an electroreduction current at a horseradish peroxidase (HRP) redox conducting hydrogel electrode poised at +100 mV (Ag/AgCl). The sensitivity of the resulting glucose sensor is 0.14A/sq cm/M with a linear range up to 2mM glucose. (jg)

Descriptors :   *CROSSLINKING(CHEMISTRY), *BIOLOGICAL DETECTION, *GLUCOSE, *VITAMIN B COMPLEX, BIOCHEMISTRY, ENZYMES, OXIDATION, ELECTRODES, CATALYSIS, ELECTRIC CURRENT, GELS, PEROXIDASES.

Subject Categories : Organic Chemistry
      Biochemistry
      Physical Chemistry
      Electricity and Magnetism

Distribution Statement : APPROVED FOR PUBLIC RELEASE