Accession Number : ADA290520
Title : Effect of Mild Hydrostatic Pressure on Red Blood Cells.
Descriptive Note : Final rept. Aug 91-Oct 94,
Corporate Author : HEBREW UNIV JERUSALEM (ISRAEL)
Personal Author(s) : Yedgar, Saul
PDF Url : ADA290520
Report Date : 26 DEC 1994
Pagination or Media Count : 5
Abstract : We studied the effect of physiological levels of hydrostatic pressure, such as that applied in diving or hyperbaric treatment, on (1) flow (rheological) properties and (2) membrane fluidity and morphology of red blood cells (RBC). It was found that application of such pressure (1) Enhances the aggregability of RBC (studied by a computerized image analysis which was developed for this purpose), and higher than normal flow rate was required to disperse the cells. (2) Reduces RBC membrane fluidity (studied by fluorescence anisotropy of lipid probes and tryptophan), and changes the cell morphology (studied by scanning electron microscopy) from normal discocytes to stomatocytes. Physical and rheological properties of RBO, as well as their shape, play a major role in blood flow and in their cellular-biochemical functions. Thus, the alterations in RBC reported here may be pertinent to the microcirculatory and physiological distorters observed among humans subjected to elevated pressure.
Descriptors : *IMAGE PROCESSING, *COMPUTER AIDED DIAGNOSIS, *BLOOD CELLS, PROBES, SCANNING ELECTRON MICROSCOPES, FLUORESCENCE, HUMANS, PHYSICAL PROPERTIES, LIPIDS, MORPHOLOGY, HIGH PRESSURE, ELECTRON MICROSCOPY, FLOW RATE, ANISOTROPY, RHEOLOGY, MEMBRANES, FLUIDS, PHYSIOLOGY, ERYTHROCYTES, DIVING, BLOOD CIRCULATION, HYPERBARIC CONDITIONS, HYDROSTATIC PRESSURE, TRYPTOPHAN.
Subject Categories : Anatomy and Physiology
Medicine and Medical Research
Distribution Statement : APPROVED FOR PUBLIC RELEASE