Accession Number : ADA290659

Title :   Subchronic Toxicity Studies on 1,3,5-Trinitrobenzene, 1,3-Dinitrobenzene, and Tetryl in Rats. Subchronic Toxicity Evaluation of N-Methyl-N, 2,4,6-Tetranitroaniline (Tetryl) in Fischer 344 Rats.

Descriptive Note : Final rept.,

Corporate Author : ENVIRONMENTAL PROTECTION AGENCY CINCINNATI OH ENVIRONMENTAL MONITORING AND SU PPORT LAB

Personal Author(s) : Reddy, Tirumuru V.

PDF Url : ADA290659

Report Date : 01 SEP 1994

Pagination or Media Count : 157

Abstract : Subchronic toxic effects of N-Methyl-N, 2,4,6-Tetranitroaniline (tetryl) in male and female Fisher 344 rats were evaluated by feeding powdered certified laboratory chow supplemented with varied concentrations of tetryl (0, 200, 1000 and 3000 mg/kg diet) for ninety days. There were no clinical signs of toxicity or early deaths. Food intake was reduced in both sexes at all dose levels throughout the study and resulted in a significant decrease in terminal body weights in the 3000 and 1000 mg tetryl dose groups. The calculated average tetryl dosage was 14, 69 and 199 mg/kg/day for females and 13, 62 and 180 mg/kg/day for males. An increase in the relative liver and kidney weights in both sexes in the 3000 and 1000 mg tetryl dose groups and an increase in the relative spleen weight in the 3000 mg dose group were noted. Hematology and clinical chemistry analyses were done at 45 and go days exposure. Methemoglobin levels were increased in both sexes in the 3000 and 1000 tetryl dose groups at 45 and 90 days while hemoglobin was decreased in these same groups at 90 days The red blood cell count was decreased in the 3000 mg tetryl dose group of both sexes while the reticulocyte count was increased in this same group. Cholesterol levels were increased in all dose groups in both sexes at 90 days. Histopathological examinations suggested that susceptible organs for tetryl toxicity were spleen (pigment deposition and erythroid cell hyperplasia) and kidneys (tubular degeneration and cytoplasmic droplets). A no observed adverse effect level (NOAEL) of 13 mg/kg/day was established.

Descriptors :   *TOXICITY, *TETRYL, CHEMICAL CONTAMINATION, RATS, SYNTHESIS(CHEMISTRY), COUNTING METHODS, CLINICAL MEDICINE, SIGNS AND SYMPTOMS, DEOXYRIBONUCLEIC ACIDS, CHEMISTRY, DEPOSITION, GROUND WATER, SOILS, DROPS, WEIGHT, ADVERSE CONDITIONS, DOSAGE, CHOLESTEROL, CONCENTRATION(COMPOSITION), LEVEL(QUANTITY), TUBULAR STRUCTURES, CYTOPLASM, MALES, LIVER, ENVIRONMENTAL PROTECTION, PIGMENTS, HEMOGLOBIN, ERYTHROCYTES, TNT, BODY WEIGHT, FEMALES, METHEMOGLOBIN, SPLEEN, ORGANS(ANATOMY), FOOD CONSUMPTION, KIDNEYS, BIODETERIORATION, HEMATOLOGY.

Subject Categories : Toxicology
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE