Accession Number : ADA290811

Title :   Enzyme Design for Nonaqueous Media: Optimization of Enzymatic Catalysis for Organic Solvent Systems.

Descriptive Note : Final rept. 15 Jul 91-14 Jul 94,

Corporate Author : IOWA UNIV IOWA CITY

Personal Author(s) : Dordick, Jonathan S. ; Clark, Douglas S.

PDF Url : ADA290811

Report Date : 14 SEP 1994

Pagination or Media Count : 5

Abstract : From a biotechnological perspective there are many potential advantages of employing enzymes in organic as opposed to aqueous media. To that end, we have concluded an initial three-year research program in the area of enzymatic catalysis in organic solvents. Our studies have focused on the effect of protein hydration on subtilisins BPN' and Carlsberg in nonaqueous media. Investigations on protein engineered mutants, catalyst engineering studies, and structural studies, primarily employing EPR spectroscopy, have revealed fundamental information on the role of water, the the nature of enzyme structure, and the effects of solvents on the catalytic activity in organic solvents. This study has also resulted in several rational methods to dramatically improve enzyme activity under anhydrous conditions. Catalytic activities in organic solvents can now be expressed at levels similar to that in water--this is a major advance in the field of nonaqueous enzymology. (jg)

Descriptors :   *ENZYMES, *CATALYSIS, *ORGANIC SOLVENTS, *DEHYDRATION, OPTIMIZATION, SPECTROSCOPY, STRUCTURAL PROPERTIES, WATER, PROTEINS, MUTATIONS, ORGANIC MATERIALS, MEDIA, CATALYSTS, DESIGN CRITERIA.

Subject Categories : Organic Chemistry
      Biochemistry
      Physical Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE