Accession Number : ADA291937
Title : Polymeric Microcapsule Arrays.
Descriptive Note : Interim rept.,
Corporate Author : COLORADO STATE UNIV FORT COLLINS DEPT OF CHEMISTRY
Personal Author(s) : Martin, Charles R. ; Parthasarathy, Ranjani V.
PDF Url : ADA291937
Report Date : 24 MAR 1995
Pagination or Media Count : 10
Abstract : Immobilized enzymes are used in bioreactors and biosensors. Current methods for immobilizing enzymes include adsorption or covalent attachment to a support, microencapsulation and entrapment within a membrane/film or gel. The ideal enzyme immobilization method would (1) Employ mild chemical conditions; (2) Allow for large quantities of enzyme to be immobilized; (3) Provide a large surface area for enzyme/substrate contact within a small total volume; (4) Minimize barriers to mass transport of substrate and product and (5) Provide a chemically and mechanically robust system. This report describes a new method for enzyme immobilization that satisfies all of these criteria. We have developed a template-based synthetic method that yields hollow polymeric microcapsules of uniform diameter and length. These microcapsules are arranged in a high density array in which the individual capsules protrude from a surface like the bristles of a brush. We have developed procedures for filling these microcapsules with high concentrations of enzymes. The enzyme-loaded microcapsule arrays function as enzymatic bioreactors in both aqueous solution and organic solvents.
Descriptors : *MOBILIZATION, *ENZYMES, *ARRAYS, DIAMETERS, MOBILITY, DETECTORS, HIGH RATE, SOLUTIONS(MIXTURES), CHEMICALS, WATER, ADSORPTION, BIOCHEMISTRY, COVALENT BONDS, FILMS, SUBSTRATES, SURFACES, CHEMICAL REACTORS, MASS TRANSFER, HIGH DENSITY, CONCENTRATION(COMPOSITION), RESTRAINT, MEMBRANES, ATTACHMENT, BIOLOGICAL DETECTION, ORGANIC SOLVENTS.
Subject Categories : Biochemistry
Distribution Statement : APPROVED FOR PUBLIC RELEASE