Accession Number : ADA291937

Title :   Polymeric Microcapsule Arrays.

Descriptive Note : Interim rept.,

Corporate Author : COLORADO STATE UNIV FORT COLLINS DEPT OF CHEMISTRY

Personal Author(s) : Martin, Charles R. ; Parthasarathy, Ranjani V.

PDF Url : ADA291937

Report Date : 24 MAR 1995

Pagination or Media Count : 10

Abstract : Immobilized enzymes are used in bioreactors and biosensors. Current methods for immobilizing enzymes include adsorption or covalent attachment to a support, microencapsulation and entrapment within a membrane/film or gel. The ideal enzyme immobilization method would (1) Employ mild chemical conditions; (2) Allow for large quantities of enzyme to be immobilized; (3) Provide a large surface area for enzyme/substrate contact within a small total volume; (4) Minimize barriers to mass transport of substrate and product and (5) Provide a chemically and mechanically robust system. This report describes a new method for enzyme immobilization that satisfies all of these criteria. We have developed a template-based synthetic method that yields hollow polymeric microcapsules of uniform diameter and length. These microcapsules are arranged in a high density array in which the individual capsules protrude from a surface like the bristles of a brush. We have developed procedures for filling these microcapsules with high concentrations of enzymes. The enzyme-loaded microcapsule arrays function as enzymatic bioreactors in both aqueous solution and organic solvents.

Descriptors :   *MOBILIZATION, *ENZYMES, *ARRAYS, DIAMETERS, MOBILITY, DETECTORS, HIGH RATE, SOLUTIONS(MIXTURES), CHEMICALS, WATER, ADSORPTION, BIOCHEMISTRY, COVALENT BONDS, FILMS, SUBSTRATES, SURFACES, CHEMICAL REACTORS, MASS TRANSFER, HIGH DENSITY, CONCENTRATION(COMPOSITION), RESTRAINT, MEMBRANES, ATTACHMENT, BIOLOGICAL DETECTION, ORGANIC SOLVENTS.

Subject Categories : Biochemistry
      Organic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE