Accession Number : ADA293241

Title :   Control of Thermophilic Spore Activity with Pressurized COSub2 and Egg-White Lysozyme.

Descriptive Note : Final rept. 30 Oct 90-30 Sep 93,

Corporate Author : ARMY NATICK RESEARCH DEVELOPMENT AND ENGINEERING CENTER MA

Personal Author(s) : Sikes, Anthony ; Martin, Cindy

PDF Url : ADA293241

Report Date : MAR 1995

Pagination or Media Count : 23

Abstract : The shelf life of low-acid (pH 5.4-5.6), thermally processed military rations, such as mixed vegetables or stews, can be limited by the metabolic activity of gram-positive, heat-resistant spore-formers, such as Bacillus stearothermophilus. For that reason, an investigation was initiated to evaluate the feasibility of using carbon dioxide (CO2) alone or in combination with egg white lysozyme to inactivate foodborne thermophilic sporeformers. Results showed that vegetative cells of B. stearothermophilus were very sensitive to hyperbaric (pressurized) CO2. During 3.5 h of incubation in AAMS broth at 25 deg C and 200 psi CO2, a 5-6 log10 cycle decrease occurred in the cell density. Similarly, at 400 psi CO2, a 6-7 log decrease occurred in the cell population. However, in the spore state, results indicated that B. stearothermophilus was more resistant to CO2 inhibition than vegetative cells.

Descriptors :   *CARBON DIOXIDE, *MURAMIDASE, *THERMOPHILIC FUNGUS, DENSITY, CELLS, ENZYMES, BACILLUS, SPORES, REDUCTION, MEDICINE, SAMPLING, MIXING, STORAGE, VEGETATION, INHIBITION, EGGS, CELLS(BIOLOGY), VEGETABLES, HEAT RESISTANT MATERIALS, MILITARY RATIONS.

Subject Categories : Biochemistry
      Biology
      Inorganic Chemistry

Distribution Statement : APPROVED FOR PUBLIC RELEASE