Accession Number : ADA294162

Title :   Cellular Approaches to the Treatment of Clinical Shock.

Descriptive Note : Technical rept. 15 Sep 94-15 Mar 95,

Corporate Author : GALILEO LABS INC SUNNYVALE CA

Personal Author(s) : Miller, Guy

PDF Url : ADA294162

Report Date : 15 APR 1995

Pagination or Media Count : 13

Abstract : A convenient model system has been developed to examine the effects of energy depletion on cells as a model of clinical shock. Cells are grown on microporous polycarbonate transwells and placed into proximity to a pH sensing silicon chip. Cellular proton flux is recorded as a function of time (extracellular acidification rate). Four cell lines were examined for their suitability in this model system: (1) liver cells; (2) kidney cells; (3) intestinal cells; and (4) tumor cells. Changes in proton flux correlated with other standard viability indices. Further work is being performed to characterize more fully this system.

Descriptors :   *CLINICAL MEDICINE, *CELLS(BIOLOGY), *CATABOLISM, DEPLETION, MILITARY PERSONNEL, TISSUES(BIOLOGY), DETECTION, MODELS, CELLS, ENERGY, NEOPLASMS, CHIPS(ELECTRONICS), PROTONS, OXYGEN, DIAGNOSIS(MEDICINE), SILICON, SHOCK, VIABILITY, MILITARY MEDICINE, INDEXES, WOUNDS AND INJURIES, LIVER, POLYCARBONATES, PH FACTOR, INTESTINES, POROUS MATERIALS, BLOOD SUBSTITUTES, HEMORRHAGIC SHOCK, KIDNEYS, PARTICLE FLUX.

Subject Categories : Medicine and Medical Research
      Stress Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE