Accession Number : ADA294245

Title :   Application of Laboratory Robotics to the Determination of the Primary Structure of Recombinant Proteins and the Measurement of Endotoxin.

Descriptive Note : Final rept. 1 Nov 89-30 Sep 94,

Corporate Author : HEALTH RESEARCH INC BUFFALO NY

Personal Author(s) : Cowens, J. W. ; Ehrke, M. J.

PDF Url : ADA294245

Report Date : 30 SEP 1994

Pagination or Media Count : 5

Abstract : The design of customized and the modification of existing standard robot hardware have been completed and the automated system is now capable of carrying out the tasks necessary to complete the amino acid analyses and protein primary sequence analyses (including protein hydrolysis and derivatization). The robotic amino acid analysis system has now been validated and shown to be capable of analyzing proteins in the 17kD range. The proposed modification of these procedures for endotoxindetectionhave been shown to be appropriate. These studies have demonstrated that once the complex series of steps involved in carrying out a chemical analytical procedure are reduced to a series of modular operations, these procedures can be performed by a robot. Once these modular operations have been automated they can be used in any configuration to carry out other defined tasks consisting of the same modular operations and with the appropriate hardware the robot can carry these procedures out on a micro scale.

Descriptors :   *ROBOTICS, *ROBOTS, *ENDOTOXINS, MEASUREMENT, DETECTION, CHEMICALS, PROTEINS, MODULAR CONSTRUCTION, SEQUENCES, SCALE, LABORATORIES, HYDROLYSIS, AMINO ACIDS.

Subject Categories : Toxicology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE