Accession Number : ADA294354

Title :   Amphetamine Redisturbutes Dopamine from Synaptic Vesicles to the Cytosol and Promotes Reverse Transport.

Descriptive Note : Technical rept.,

Corporate Author : PENNSYLVANIA STATE UNIV UNIVERSITY PARK DEPT OF CHEMISTRY

Personal Author(s) : Sulzer, D. ; Chen, T. K. ; Lau, Y. Y. ; Kristensen, H. H. ; Rayport, S.

PDF Url : ADA294354

Report Date : 10 OCT 1994

Pagination or Media Count : 31

Abstract : Whether amphetamine acts principally at the plasma membrane or at synaptic vesicles is controversial. We find that d-amphetamine injection into the Planorbis giant dopamine neuron causes robust dopamine release, demonstrating that specific amphetamine uptake is not required. Arguing for action at vesicles, whole cell capillary electrophoresis of single Planorbis dopamine neurons shows that amphetamine reduces vesicular dopamine, while amphetamine reduces quantal dopamine release from Pc12 cells by >50% per vesicle. Intracellular injection of dopamine into the Planorbis dopamine neuron produces rapid nomifensine-sensitive release, showing that an increased substrate concentration gradient is sufficient to induce release. These experiments indicate that amphetamine acts at the vesicular level where it redistributes dopamine to the cytosol, promoting reverse transport and dopamine release.

Descriptors :   *INJECTION, *NEUROTRANSMITTERS, *DOPAMINE, CELLS, PLASMAS(PHYSICS), SUBSTRATES, REVERSIBLE, TRANSPORT, CONCENTRATION(COMPOSITION), RELEASE, NERVE CELLS, MEMBRANES, CELLS(BIOLOGY), ELECTROPHORESIS, CAPILLARITY, GRADIENTS, AMPHETAMINES.

Subject Categories : Physical Chemistry
      Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE