Accession Number : ADA294612

Title :   Chemical Blistering: Cellular and Macromolecular Components.

Descriptive Note : Final rept. 1 Jun 90-31 Oct 94,

Corporate Author : MICHIGAN UNIV ANN ARBOR

Personal Author(s) : Bernstein, I. A.

PDF Url : ADA294612

Report Date : 31 OCT 1994

Pagination or Media Count : 81

Abstract : The mission of this project was to determine the cellular and molecular lesions associated with cutaneous vesication from bis(2-chloroethyl)sulfide (BCES). Cultures of keratinocytes were used to focus attention on the direct interactions between the mustard and its epidermal targets. The technical objectives included confirming that DNA was the primary molecular target of BCES in human epidermal keratinocytes, identifying and quantifying BCES- mediated DNA-adducts in relation to dose, determining why epidermal basal cells are more susceptible to BCES than differentiated cells, and investigating the possible role of informational error in DNA in the cytopathogenic process. Data generated in the project suggest that (a) DNA is the primary epidermal target of BCES, and the fidelity of DNA repair governs survival of the germinative population, and (b)BCES causes a decrease in the germinative population by cellular differentiation, as indicated by the appearance of mature keratin protein, as well as necrosis.

Descriptors :   *DEOXYRIBONUCLEIC ACIDS, *CHEMICAL AGENTS, *ALKYLATION, *CHEMICAL INDICATORS, INTERACTIONS, MOLECULES, TARGETS, SURVIVAL(GENERAL), REPAIR, ERRORS, MACROMOLECULES, PATHOLOGY, CELLS(BIOLOGY), POROUS MATERIALS, LESIONS, NECROSIS, VESICANTS, SKIN DISEASES, TISSUE CULTURE.

Subject Categories : Chemical, Biological and Radiological Warfare

Distribution Statement : APPROVED FOR PUBLIC RELEASE