Accession Number : ADA295696

Title :   Molecular Ecology of Bacterial Populations in Environmental Hazardous Chemical Control.

Descriptive Note : Final rept. 15 Jan 92-14 May 95,

Corporate Author : TENNESSEE UNIV KNOXVILLE CENTER FOR ENVIRONMENTAL BIOTECHNOLOGY

Personal Author(s) : Sayler, Gary S.

PDF Url : ADA295696

Report Date : 14 MAY 1995

Pagination or Media Count : 106

Abstract : The correlation between bioavailability and biodegradative capability in the environment has always been a puzzle for bioremediation. Furthermore, the detection of I biodegradative activities in situ also has hampered biological site characterization. All of these due to lack of proper tool(s) or method(s) that can be applied readily, specifically, and feasibly to the environmental pollutants. However, the development and application of bioluminescent reporter strains for continuously real-time monitoring the relationship between bacterial degradative activities and bioavailability of environmental pollutants were examined in this study. The results obtained from this investigation suggested that bioluminescent reporters can provide continuous, and precise insight information onboth molecular and physiological level. The more important is that these bioreporters will not interrupt and complete with indigenous bacteria. The versatility of the catabolic capability on the degradation of different higher molecular PAHs by a NAH plasmid-mediated metabolism was also examined. The results obtained in this study indicated that the NAH plasmid plays an important role-on the biodegradation of PAHs Furthermore, the naphthalene degradation pathways serves an essential route for the study of bacterial degradation pathway on PAHs. jg

Descriptors :   *ENVIRONMENTS, *HAZARDS, *CHEMICALS, *MOLECULES, *BACTERIA, *PLASMIDS, *ECOLOGY, *NAPHTHALENES, CONTROL, DETECTION, DEGRADATION, BIOLOGY, MONITORING, REAL TIME, MOLECULAR STATES, SITES, POPULATION, ORGANIC MATERIALS, PHYSIOLOGY, POLLUTANTS, CONTINUITY, BIODEGRADATION, CATABOLISM.

Subject Categories : Ecology
      Biology
      Inorganic Chemistry
      Organic Chemistry
      Physical Chemistry
      Environmental Health and Safety

Distribution Statement : APPROVED FOR PUBLIC RELEASE