Accession Number : ADA296019

Title :   Differential Gene Expression in Mammalian Liver During Hibernation.

Descriptive Note : Rept. for 1 Feb 92-31 Mar 95,

Corporate Author : COLORADO UNIV HEALTH SCIENCES CENTER DENVER

Personal Author(s) : Martin, Sandra L.

PDF Url : ADA296019

Report Date : 23 MAY 1995

Pagination or Media Count : 6

Abstract : Hibernation in mammals lead to profound reductions in metabolic, heart, and respiratory rates, as well as body temperature. The physiological events that lead to hibernation are precisely controlled, yet little is known about how these events are orchestrated at the molecular level. The first step toward a detailed molecular understanding of hibernation is to isolate and identify gene products that are uniquely expressed during hibernation. Liver was chosen to begin such a study because of its primary role in homeostasis; this role is likely to be critical during the physiological extremes experienced during hibernation. We used both a specific and a general approach to examine liver gene expression during hibernation. Alpha-2-macroglobulin, a broad-spectrum protease inhibitor, is expressed at elevated levels during hibernation. Because alpha- 2-macroglobulin is an acute phase reactant in some species, we asked whether the acute phase response of the liver is a normal part of the biochemical events necessary for hibernation. In order to examine the general features of differential gene expression in the liver during hibernation, we used a subtractive hybridization approach to isolate and characterize cDNAs corresponding to genes that are induced in the hibernating state.

Descriptors :   *MAMMALS, *GENES, *LIVER, *HIBERNATION, MOLECULAR STATES, BIOCHEMISTRY, RATES, SPECTRA, RESPONSE, BROADBAND, RESPIRATORY SYSTEM, PHYSIOLOGY, INHIBITORS, HOMEOSTASIS, PEPTIDE HYDROLASES, BODY TEMPERATURE, HYBRIDIZATION.

Subject Categories : Ecology
      Biology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE