Accession Number : ADA296837

Title :   Observations of a Circular, Triple-Helical Polysaccharide using Non-Contact Atomic Force Microscopy.

Descriptive Note : Interim rept. May 94-Jun 95,

Corporate Author : CALIFORNIA UNIV IRVINE DEPT OF CHEMISTRY

Personal Author(s) : McIntire, Theresa M. ; Penner, Reginald M. ; Brant, David A.

PDF Url : ADA296837

Report Date : 30 JUN 1995

Pagination or Media Count : 13

Abstract : The non-contact atomic force microscopy (NC-AFM) technique is applied to achieve molecular resolution of the immunostimulatory (1->8)-branched b(1->3)glucan scleroglucan. Sample preparation involves deposition of the polysaccharide molecules from an aqueous solution onto a freshly cleaved and atomically smooth mica surface by spraying and air drying. High resolution NC-AFM images have been obtained in air from which the mean polymer chain length (and molar mass) and the mean chain diameter have been estimated from measurements on a sizable sample of molecules. jg p.2

Descriptors :   *POLYSACCHARIDES, DIAMETERS, SOLUTIONS(MIXTURES), AIR, MOLECULES, WATER, POLYMERS, HIGH RESOLUTION, MICROSCOPY, SURFACES, DEPOSITION, LENGTH, CHAINS, IMAGES, MEAN, MOLECULAR PROPERTIES, DRYING, MICA.

Subject Categories : Polymer Chemistry
      Biochemistry
      Organic Chemistry
      Optics

Distribution Statement : APPROVED FOR PUBLIC RELEASE