Accession Number : ADA297025

Title :   In Vitro Systems for Studying Metabolism of Environmental Chemicals in Human Cells.

Descriptive Note : Final rept. 30 Apr 91-29 Apr 95,

Corporate Author : SRI INTERNATIONAL MENLO PARK CA

Personal Author(s) : Green, C. E.

PDF Url : ADA297025

Report Date : 25 JUL 1994

Pagination or Media Count : 24

Abstract : The objective of the project is to establish and use an in vitro system of intact isolated cells from rodent and human tissues to develop quantitative data on the metabolism of toxic chemicals that can be used for risk assessments. The following halogenated aliphatic solvents are being studied: chloroform, 1,1, 1-trichloroethane, trichloroethylene, dicloromethane, bromochloromethane, and carbon tetrachloride. Chloroform is being used to establish the conditions with rat liver preparations for the generation of kinetic constants for metabolism, which are determined as disappearance- of the parent compound. Isolated hepatocytes and precision-cut liver slices are being compared to optimize the correspondence between the in vitro results obtained and the published in vivo data. The system developed with rat liver is now being applied to human liver incubations. The same set of halogenated solvents is being studied, and the resulting data should allow quantitative comparison of the metabolism and cytotoxicity in these species. These data will be analyzed to characterize interspecies differences in the kinetics of metabolism.

Descriptors :   *IN VITRO ANALYSIS, *CELLS(BIOLOGY), *CULTURES(BIOLOGY), TEST AND EVALUATION, TISSUES(BIOLOGY), ENVIRONMENTS, RISK, RATS, HUMANS, CHEMICALS, CELLS, TOXICITY, ISOLATION, ALIPHATIC COMPOUNDS, METABOLISM, YIELD, SOLVENTS, KINETICS, SUSPENSION DEVICES, SULFATES, LIVER, HALOGENATION, IN VIVO ANALYSIS, ANTIBIOTICS, TRICHLOROETHYLENE, ANTIINFLAMMATORY AGENTS, BIOPSY, INSULIN, SERINE, THYROXINE, ALANINES, OLEIC ACID, CARBON TETRACHLORIDE, GLUCAGON, TESTOSTERONE.

Subject Categories : Anatomy and Physiology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE