Accession Number : ADA297172

Title :   The Effects of Signaling Through the EGF Receptor System Upon Regulation of Growth in Human Mammary Epithelial Cells.

Descriptive Note : Annual rept. 15 May 94-14 May 95,

Corporate Author : UTAH UNIV SALT LAKE CITY

Personal Author(s) : Worthylake, Becky

PDF Url : ADA297172

Report Date : 31 MAY 1995

Pagination or Media Count : 17

Abstract : The levels of the Human EGF Receptor (HER1) and the related receptors HER2,3, and 4 were examined in human-mammary epithelial cells (HMEC's). When HMEC's are grown on plastic tissue culture dishes HER11 is expressed at high levels, while HER2 and HER4 are only expressed at low levels. No HER3 was detected. The expression of the various ligands to these receptors was examined. HMEC's express high levels of amphiregulin; moderate levels of TGF-alpha, HB EGF; and very low level of EGF and B celluin. The effects of HER1 stimulation on the levels of HER2 were examined in both IlMEC's and mouse fibroblasts. Addition of exogenous EGF leads to a decrease in the levels of HER2. This down regulation occurs in 3 hours, this, the mechanism appears to be post transcriptional

Descriptors :   *EPITHELIUM, *CANCER, *MAMMARY GLANDS, CONTROL, HUMANS, GROWTH(GENERAL), LIGANDS, LOW LEVEL, FIBROBLASTS, MICE, PLASTICS, SENSE ORGANS, TISSUE CULTURE.

Subject Categories : Anatomy and Physiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE