Accession Number : ADA297181

Title :   Molecular Cloning and Function of Fas/APO-1 Associated Protein in Breast Cancer.

Descriptive Note : Annual rept. 9 May 94-8 May 95,

Corporate Author : LA JOLLA CANCER RESEARCH FOUNDATION CA

Personal Author(s) : Sato, Takaaki ; Reed, John

PDF Url : ADA297181

Report Date : 05 JUN 1995

Pagination or Media Count : 53

Abstract : Fas/APO-1 is a cell surface receptor that controls a poorly understood signal transduction pathway leading to cell death via apoptosis. A protein tyrosine phosphatase, FAP-1 (Fas/APO-1 associated phosphatase), capable of interacting with the cytosolic domain of Fas, was isolated by yeast two hybrid system. The carboxy terminal 15 amino acids of Fas are necessary and sufficient for interaction with FAP-1. PAP-i expression is highest in tissues and cell lines (including MCF7 breast cancer cell line) that are relatively resistant to Fas-mediated cytotoxicity. Gene transfer- mediated;elevations in FAP-1 partially abolished Fas-induced apoptosis in a T-cell line. These findings are consistent with an inhibitory effect of FAP- 1 on Fas-signal transduction.

Descriptors :   *CLONES, *CANCER, *MAMMARY GLANDS, MOLECULES, PROTEINS, SURFACES, SIGNALS, HYBRID SYSTEMS, YEASTS, GENES, TRANSDUCERS, TRANSFER, T LYMPHOCYTES, GENETIC ENGINEERING, INHIBITION, DEATH, CELLS(BIOLOGY), SENSE ORGANS, TYROSINE, PHOSPHATASES.

Subject Categories : Medicine and Medical Research
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE