Accession Number : ADA298070

Title :   The Role of Gap-Junctional Communication in Chemical Toxicology.

Descriptive Note : Final rept. 15 May 92-15 Jul 95,

Corporate Author : MICHIGAN STATE UNIV EAST LANSING

Personal Author(s) : Trosko, James E.

PDF Url : ADA298070

Report Date : 17 JUL 1995

Pagination or Media Count : 18

Abstract : The overall goal of this research project was to study the mechanisms by which non-genotoxic chemicals induced multiple disease end points such as birth defects, tumor promotion, reproductive- and neuro-toxicities. The working hypothesis was that these non-genotoxic chemicals disrupted homeostatic control of cell proliferation, differentiation and adaptive responses of differentiated cells. Specifically, to test this hypothesis, gap junctional intercellular communication (GJIC) was studied. During this grant period, several techniques were developed and applied to study how various model non-genotoxic chemicals, as well as various oncogenes, interfered with GJIC to cause abnormal cell growth and differentiation. The molecular biology, biochemistry and cell biology of non-genotoxic chemical and oncogene interference with GJIC was studied using in vitro techniques, primarily with fluorescent detection of ions and molecules via laser-assisted image analyses. jg p.1

Descriptors :   *CHEMICALS, *TOXICOLOGY, IONS, MEASUREMENT, DETECTION, FLUORESCENCE, MOLECULES, TOXICITY, BIOCHEMISTRY, DISEASES, NEOPLASMS, MUTATIONS, IN VITRO ANALYSIS, LASER APPLICATIONS, RESPONSE, ADAPTIVE SYSTEMS, MOLECULAR BIOLOGY, HYPOTHESES, INHIBITION, CELLS(BIOLOGY), MOLECULAR PROPERTIES, GENETICS, ABNORMALITIES, CANCER, HOMEOSTASIS, BIRTH, REPRODUCTIVE SYSTEM.

Subject Categories : Toxicology
      Biochemistry
      Genetic Engineering and Molecular Biology
      Atomic and Molecular Physics and Spectroscopy

Distribution Statement : APPROVED FOR PUBLIC RELEASE