Accession Number : ADA298301

Title :   Epidemiology of Enterotoxigenic E. coli (ETEC) Diarrhea in Alexandria, Egypt.

Descriptive Note : Technical rept.,

Corporate Author : NAVAL MEDICAL RESEARCH UNIT NO 3 CAIRO (EGYPT) DEPT OF MEDICAL ZOOLOGY

Personal Author(s) : Abu-Elyazeed, R. R. ; Churilla, A. M. ; Kay, B. A. ; Wierzba, T. ; Peruski, L.

PDF Url : ADA298301

Report Date : MAR 1995

Pagination or Media Count : 107

Abstract : During November 1993 - October 1994, a cohort of newborns and children under the age of two years was assembled in three phases, corresponding to these villages (village 800, village 806 and village 807) within the Abees field site. A total of 129 subjects was enrolled in the study during that period. Follow-up visits for each child occurred twice weekly and rectal swabs and stool specimens were obtained if the child had diarrheal symptoms. Rectal swabs were placed in Cary-Blair transport medium in the field and transported directly to the laboratory. In the laboratory, the swabs were plated on Macconkey's media, and 5 typical lactose-positive colonies were evaluated for both LT and ST using GMl ELISA. Colonies that were toxin-positive were evaluated for CFA/I, CFA/II, CFA/III and CFA/IV by slide agglutination using monoclonal or polyclonal sera against the colonization factors and their subtypes.

Descriptors :   *SIGNS AND SYMPTOMS, *ESCHERICHIA COLI, *DIARRHEA, *CHILDREN, EGYPT, ENZYMES, SITES, MONOCLONAL ANTIBODIES, INFECTIOUS DISEASES, MEDICAL SERVICES, IMMUNOASSAY, COLONIES(BIOLOGY), VILLAGES, AGGLUTINATION.

Subject Categories : Medicine and Medical Research
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE