Accession Number : ADA298413

Title :   Stromal Influence on Breast Cancer Progression.

Descriptive Note : Annual rept. 15 Jun 94-14 Jun 95,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : McLeskey, Sandra W.

PDF Url : ADA298413

Report Date : 14 JUL 1995

Pagination or Media Count : 58

Abstract : Metastatic estrogen receptor positive breast carcinoma may be treatable with tamoxifen, an antiestrogen, but the tumor may subsequently become refractory to treatment, growing in the absence of estrogenic stimulation. We have developed a model of breast cancer progression by transfecting MCF-7 breast carcinoma cells, which are estrogen-dependent for growth in ovariectornized nude mice, with cDNAs for FGF-l or FGF-4. he transfected cell lines are able to form tumors in ovariectomized nude mice. Since FGFs are angiogenic actors, this project investigates the importance of angiogenesis in the phenotypic transition of the transfected cells by looking for differences in the angiogenesis in tumors produced by the parental MCF-7 or the FGF- transfected cells. Our model is validated by a positive correlation of tumor microvessel density and tumor size. We have defined temporal and spatial events in the process of tumor-induced angiogenesis by identifing sprouting or proliferating endothelial cells. We have identified patterns of angiogenesis which are associated with regressing parental cell tumors and growing FGF-transfected cell tumors. (AN)

Descriptors :   *FIBROBLASTS, *CANCER, *MAMMARY GLANDS, STIMULATION(PHYSIOLOGY), NEOPLASMS, DEOXYRIBONUCLEIC ACIDS, IN VITRO ANALYSIS, CYTOPLASM, GENES, CELL DIVISION, MICE, CARCINOGENS, MONOCLONAL ANTIBODIES, MEDICAL RESEARCH, IN VIVO ANALYSIS, RECEPTOR SITES(PHYSIOLOGY), BLOOD VESSELS, LESIONS, GROWTH(PHYSIOLOGY), IMMUNOCHEMISTRY, ESTROGENS, METASTASIS, OVARIES.

Subject Categories : Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE