Accession Number : ADA298703

Title :   Spatial Distribution of the EGF Receptor System in the Regulation of Breast Epithelia Cell Growth and Organization.

Descriptive Note : Annual rept. May 94-May 95,

Corporate Author : UTAH UNIV SALT LAKE CITY

Personal Author(s) : Burke, Patrick

PDF Url : ADA298703

Report Date : 12 JUN 1995

Pagination or Media Count : 10

Abstract : 184A1 human mammary epithelial cells (HMECs) form organotypic structures when plated on Matrigel. However, the organotypic structures differentiate into squamous, not glandular, epithelia. They can not be used to examine the functional significance of a polarized epidermal growth factor receptor system in a glandular epithelia. This experimental system may be useful for investigating cell-extracellular matrix interactions that control EGF-R expression. 184A1s and subclones of the 184A1 cell line express proteins necessary for structural and functional polarization on Transwells, but do not assemble these proteins into tight junctions. MCF-7 cells express ZO-1 and assemble the protein into tight junctions when plated onto Transwells. MCF-7s also segregate B-1 integrin into the basal-lateral membrane coppartment. NCF-7 cells respond mitogenicly to EGF-R occupancy and may provide an experimental system with which to address the primary aim of my proposal: to determine if the loss of the correct spatial organization or inappropriate expression of the EGF-R system provides a growth advantage, enhances cell motility, or ochanges the differentiated state of normally organized epithelial cells.

Descriptors :   *EPITHELIUM, *CANCER, *MAMMARY GLANDS, SPATIAL DISTRIBUTION, POLARIZATION, ORGANIZATIONS, STRUCTURAL PROPERTIES, PROTEINS, DISEASES, SENSE ORGANS, TIGHTNESS.

Subject Categories : Anatomy and Physiology
      Medicine and Medical Research

Distribution Statement : APPROVED FOR PUBLIC RELEASE