Accession Number : ADA298704

Title :   Structure and Function of the Ankyrin Repeats in the SWI4/SWI6 Transcription Complex of Budding Yeast.

Descriptive Note : Annual rept. 8 Jul 94-7 Jul 95,

Corporate Author : FRED HUTCHINSON CANCER RESEARCH CENTER SEATTLE WA

Personal Author(s) : Breeden, Linda

PDF Url : ADA298704

Report Date : 08 AUG 1995

Pagination or Media Count : 14

Abstract : The intent of this research is to define the structure and function of a highly conserved protein motif whose ectopic expression causes leukaemia, lymphoma, and mammary carcinoma. This protein motif, called an ankyrin or ANK repeat has been found in a diverse set of proteins in bacteria, plants, fungi, insects, and vertebrates. The ANK repeat is 33 amino acids long and is always tandemly repeated. The analysis that has been carried out indicates that the ANK repeats are required for the activity of the proteins in which they reside, but little progress has been made in defining their function. Deletions within the ANK repeat domains of several proteins suggest that the repeat may be involved in protein:protein interaction. We are identifying the critical residues and the biological function of the ANK repeats found in two yeast transcription factors. Using yeast allows us to simultaneously exploit genetic screens, gene replacement, biochemistry and immunochemistry in this analysis. A thorough investigation of these repeats in a simple system will provide insight and testable predictions which will augment and expedite their analysis in higher cell ANK proteins. It will also provide valuable reagents for identifying other ANK repeat proteins and defining their functions.

Descriptors :   *BACTERIA, *YEASTS, *LYMPHOMAS, PREDICTIONS, INTERACTIONS, CELLS, BIOCHEMISTRY, PROTEINS, TEST METHODS, VERTEBRATES, REPLACEMENT, GENES, RESIDUES, INSECTS, IMMUNOCHEMISTRY.

Subject Categories : Anatomy and Physiology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE