Accession Number : ADA298926

Title :   Genetic Toxicity Evaluation of Iodotrifluoromethane (CFsub3I). Volume 1. Results of Salmonella Typhimurium Histidine Reversion Assay (Ames Assay).

Descriptive Note : Final rept. Mar-Dec 94,

Corporate Author : MANTECH ENVIRONMENTAL TECHNOLOGY INC DAYTON OH

Personal Author(s) : Mitchell, A. D.

PDF Url : ADA298926

Report Date : JAN 1995

Pagination or Media Count : 24

Abstract : Under subcontract to ManTech Environmental Technology, Incorporated, Genesys Research, Incorporated, tested lodotrifluoromethane (CF3I) using Billups-Rothenberg exposure chambers for the exposure chamber modification of the Salmonella typhimurium histidine (his) reversion mutagenesis system (the Ames test), a microbial assay that measured histidine + reversion induced by chemicals that cause base changes or frameshift mutations in the genome of this organism. Testing was conducted using five Salmonella strains, with and without metabolic activation. CF3I was treated in a preliminary test, which was repeated because of excessive toxicity and in a mutagenesis test. When tested over a concentration range of 1,060 to 85,908 ppm, CF3I was found to be highly mutagenic, inducing frameshift and, particularly, base-pair mutations in Salmonella typhimurium, without and with activation. Therefore, CF3I was positive in the Salmonella typhimurium histidine (his) reversion mutagenesis assay.

Descriptors :   *TOXICITY, *SALMONELLA TYPHIMURIUM, TEST AND EVALUATION, ACTIVATION, ENVIRONMENTS, EXPOSURE(GENERAL), METABOLISM, MUTATIONS, STRAINS(BIOLOGY), GENETICS, CHAMBERS, MICROORGANISMS, HISTIDINE.

Subject Categories : Microbiology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE