Accession Number : ADA299032

Title :   Range-Finding Study for a Reproductive Assessment of 1,3,5- Trinitrobenzene Administered in the Diet of Sprague-Dawley Rats.

Descriptive Note : Final rept. Nov 92-May 93,

Corporate Author : MANTECH ENVIRONMENTAL TECHNOLOGY INC DAYTON OH

Personal Author(s) : Kinkead, E. R. ; Wolfe, R. E. ; Salins, S. A. ; Flemming, C. D. ; Caldwell, D. J.

PDF Url : ADA299032

Report Date : APR 1994

Pagination or Media Count : 41

Abstract : The soil and groundwater of several military installations targeted for restoration contain measurable quantities of 1,3,5- trinitrobenzene (TNB). As part of the process to develop environmental and health effects criteria, a single-generation reproduction study was performed. Male and female Sprague-Dawley rats received diet containing approximately 800, 400, or 70 mg TNB/kg diet throughout the study. Mating occurred following 14 days of treatment. Dams and pups were maintained through 21-days postpartum while male rats were necropsied following 35 treatment days. No mortality occurred in parental animals during the study. A treatment-related decrease in food consumption occurred in both sexes with a concurrent depression of body weight gain. Mean testes weights of the high-and mid-dose rats were significantly less than control testes weights. Likewise, epididymides of test animals weighed significantly less than those of controls. Histopathologic examinations showed testicular degeneration in 100% of the high-dose dams and 70% of the mid-dose animals. Sperm depletion was evident in the lumen of these same groups. All high- dose dams and one mid-dose dam displayed signs of neurotoxicity, primarily head tilt and loss of equilibrium, during the postpartum time period. Histopathologic examinations of brain samples from these animals showed vacuolated neuropil in the ovilary deep cerebellar, and vestibular nuclei. Mating indices were normal, however 4- and 21-day pup survival rates were significantly less in the high-dose group of animals.

Descriptors :   *TOXICITY, *REPRODUCTION(PHYSIOLOGY), TEST AND EVALUATION, MILITARY FACILITIES, ENVIRONMENTS, BRAIN, RATS, HEALTH, RATES, SURVIVAL(GENERAL), ENVIRONMENTAL IMPACT, GROUND WATER, GAIN, DOSAGE, NERVOUS SYSTEM, ANIMALS, MALES, LOSSES, MEAN, RANGE FINDING, DIET, TILT, BODY WEIGHT, FEMALES, NITROBENZENES, VESTIBULAR APPARATUS, NUCLEI, FOOD CONSUMPTION, DAMS, BIODETERIORATION, TESTES.

Subject Categories : Anatomy and Physiology
      Toxicology

Distribution Statement : APPROVED FOR PUBLIC RELEASE