Accession Number : ADA299033

Title :   Genetic Toxicity Evaluation of Iodotrifluoromethane (CF3I). Volume 3. Results of the Forward Mutation Assay Using L5178Y Mouse Lymphoma Cells.

Descriptive Note : Final rept. Mar-Dec 94,

Corporate Author : MANTECH ENVIRONMENTAL TECHNOLOGY INC DAYTON OH

Personal Author(s) : Mitchell, A. D.

PDF Url : ADA299033

Report Date : JAN 1995

Pagination or Media Count : 23

Abstract : Under subcontract to ManTech Environmental Technology, Incorporated, Genesys Research, Incorporated, used LSi?8Y mouse lymphoma cells from clone 3.7.2C to assess the capability of iodotrifluoromethane (CF3I) to induce gene and chromosomal mutations at the thymidine kinase (tk) locus in the absence and presence of exogenous S9 metabolic activation. To test this volatile material, cell cultures were placed in 15 ml round-bottom glass blood tubes sealed with serum stoppers, and, using a syringe, predetermined volumes of air were removed from each tube and nominal concentrations of CF3I were introduced. Three tubes were used for each concentration, a tube containing 5 ml of cells in media without metabolic activation, a tube containing 5 ml of medium. After a four-hour exposure period at 370C, the sham tubes were allowed to cool to room temperature, and the concentrations of the test material were measured using infrared (IF) analysis. CF3I was tested in two preliminary concentration range-finding assays and one mutagenesis assay, with each assay conducted in the absence and presence of metabolic activation. Contrary to information in the provided MSDS that CF3I was insoluble in water, CF3I appeared to be soluble in cell culture media because concentration-related increases in toxicity were obtained in each assay.

Descriptors :   *TOXICITY, *MUTATIONS, *GENETIC ENGINEERING, *LYMPHOMAS, TEST AND EVALUATION, FREQUENCY, FORWARD AREAS, ACTIVATION, ENVIRONMENTS, WATER, EFFICIENCY, COOLING, METABOLISM, CHROMOSOMES, ROOM TEMPERATURE, CLONES, CELLS(BIOLOGY), GENETICS, ACCEPTABILITY, CULTURES(BIOLOGY), CULTURE MEDIA, LOCUS.

Subject Categories : Toxicology
      Genetic Engineering and Molecular Biology

Distribution Statement : APPROVED FOR PUBLIC RELEASE