Accession Number : ADA299336

Title :   Lethality after Intratracheal Challenge of SEB (Staphylococcal enterotoxin B) in Dutch Rabbits.

Descriptive Note : Final rept.,

Corporate Author : ARMY MEDICAL RESEARCH INST OF INFECTIOUS DISEASES FORT DETRICK MD

Personal Author(s) : Liu, Ching-Tong ; Creasia, Donald A. ; Walters, Dianne M. ; Taylor, Thomas J.

PDF Url : ADA299336

Report Date : 14 JUN 1995

Pagination or Media Count : 24

Abstract : Selecting an animal species for studying SEB-induced toxicity has been difficult due to highly variable inter-species susceptibility. Approximately 20 years ago, non-human primates exposed intravenously or by inhalation of SEB were commonly used. Unfortunately, experiments with primates have been restricted because of limited toxin (SEB) supplies, as well as the high costs and animal use considerations for non-human primate studies. The purpose of this study was to develop techniques for inducing SEB toxicity from a pulmonary origin by direct intratracheal (IT) instillation in Dutch rabbits. Four doses of SEB (0.1, 0.3, 1.0, and 3.0 mg/kg, N = 3-9/group) were administered. Body weights and rectal temperatures were measured at 4-5 hr intervals after IT SEB injection for 40 and 80 hr, respectively. A trend of initially elevated rectal temperature over 20 hr was observed, and body weights decreased significantly 20 hr after IT SEB injection (0.3 - 3.0 mg/kg). The LD50 was determined to be 0.46 mg/kg (N = 30) with 95% confidence limits (0.18 to 1.00 mg/kg). These results imply that: (1) the Dutch rabbit is acceptable for studying SEB kinetics after IT instillation, and (2) the IT SEB instilled rabbit may be appropriate for quantitative evaluations of immunological or/and pharmacological interventions during SEB toxemia, originated from a pulmonary route.

Descriptors :   *ENTEROTOXINS, *LETHALITY, *STAPHYLOCOCCUS, TEST AND EVALUATION, TEMPERATURE, HIGH COSTS, HUMANS, TOXICITY, DOSAGE, ANIMALS, PRIMATES, BODY WEIGHT, TOXINS AND ANTITOXINS, RABBITS, INHALATION, RECTUM, TOXEMIA.

Subject Categories : Toxicology
      Microbiology

Distribution Statement : APPROVED FOR PUBLIC RELEASE