Accession Number : ADA299341

Title :   Breast Cancer Cell Metabolism Studied by MRS.

Descriptive Note : Annual rept. 1 Jul 94-30 Jun 95,

Corporate Author : GEORGETOWN UNIV WASHINGTON DC

Personal Author(s) : Cohen, Jack S.

PDF Url : ADA299341

Report Date : 30 JUL 1995

Pagination or Media Count : 24

Abstract : Many breast tumors progress from estrogen-dependent growth to a more malignant phenotype, characterized by estrogen-independence, antiestrogen resistance, and high metastatic potential. Utilizing 31P NMR spectroscopy, we have investigated this transition utilizing a series of cell lines selected from MCF-7 that reflect characteristics of the progressed phenotype, i.e. hormone-independent (MIII; LCC1), antiestrogen-resistant (LCC2; LY-2), and increased metastatic potential (MIII; LCC1; LCC2). No metabolic changes were found that associated with the metastatic phenotype. However, whilst estrogen treatment produced no consistent spectral changes in any of the cell lines, MIII cells responded to Tamoxifen treatment by consistently increasing several resonances 30%-40% above baseline values. To further understand this finding we are studying the effects of 8- cis-retinoic acid and ICI 182,780, a pure estrogen receptor antagonist. To see the effects of the hormones we are carrying out experiments with cells proliferating in Matrigel, a natural basement membrane,, and we are also testing gas impermeable tubing to assess the effects of oxygen level. We have also written a major article entitled "A History of Biological Applications of NMR" containing over 400 references, for the 50th anniversary of the discovery of the NMR phenomenon.

Descriptors :   *NUCLEAR MAGNETIC RESONANCE, *SPECTROSCOPY, *VALUE, *CANCER, *TUBES, *MAMMARY GLANDS, *ESTROGENS, HORMONES, MEMBRANES(BIOLOGY), PERMEABILITY, BIOLOGY, CELLS, NEOPLASMS, METABOLISM, GASES, OXYGEN, PURITY, SPECTRA, BASE LINES, HISTORY, SENSE ORGANS.

Subject Categories : Anatomy and Physiology
      Atomic and Molecular Physics and Spectroscopy

Distribution Statement : APPROVED FOR PUBLIC RELEASE